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Classical and Molecular Methods Used for Antiviral Drug Discovery

Classical and Molecular Methods Used for Antiviral Drug Discovery

  • Outline:

  • Only 10 of the more than 220 viruses known to infect humans now have clinically licensed antiviral drugs. While vaccine development is continuing, the SARS-CoV-2 outbreak has highlighted the crucial need for substances that can be promptly mobilized for the treatment of re-emerging or developing viral illnesses. We will address the classical and molecular methodologies utilized throughout the preclinical antiviral drug development process in this session.

  • This includes:
  • 1- Tissue culture and MTT assay to investigate the cytotoxicity of any compound
  • 2- Mode of action mechanism of antiviral activity by using Plaque assay or CPE inhibition assay
  • 3- Real time PCR to investigate the antiviral activity
  • 4- Real time PCR to investigate viral or cellular gene expression
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  • Instructor(s):
  • - Associate Prof. Abdou Kamal Allayeh
  • Associate Professor of Virology, National Research Centre, Egypt.
  • Virology & Molecular Biology Section Head, Nawah-Scientific, Egypt
  • Former postdoctoral researcher at the University of Zurich in Switzerland and the François-Rabelais University in France
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  • Duration: 1 day (6 h)
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  • Fees: 1000 LE.
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  • Min and max number of attendees:
  • Min: 5
  • Max: 15
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  • Date: 13/11/2023  from 10:00 am to 4:00 pm
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  • Location: Nawah Scientific

    (Almokattam mall, street 9, 2nd floor, Almokattam, Cairo, Egypt.)

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